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KMID : 1094720060110040282
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Biotechnology and Bioprocess Engineering
2006 Volume.11 No. 4 p.282 ~ p.287
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Purification and characterization of a recombinant Caulobacter crescentus epoxide hydrolase
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Hwang Seung-Ha
Hyun Hye-Jin
Lee Byoung-Ju
Park Young-Seub
Lee Eun-Yeol
Choi Cha-Yong
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Abstract
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ACaulobacter crescentus epoxide hydrolase (CCEH) from a recombinantEscherichia coli was purified to homogeneity using a three-step procedure. The CCEH protein was purified 7.3-fold with a 22.9% yield in overall activity. The optimal reaction temperature and pH were determined to be 37°C and pH 8.0, respectively. The addition of 10% (v/v) dimethylsulfoxide as a cosolvent improved the enantioselectivity of CCEH for a batch kinetic resolution of racemic indene oxide.
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KEYWORD
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chiral styrene oxide, chiral indene oxide, Caulobacter crescentus , epoxid hydrolase, enzyme purification
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